Nabizadeh Nodehi R, Golpayegani A, Douraghi M, Alimohammadi M, Rezaei F. Novel application of in vitro disinfection for modeling the biofilm formation inhibition, antimicrobial susceptibility and antibiotic resistance of Pseudomonas aeruginosa: a study of free and combined chlorine compounds. J Environ Health Sci Eng. 2022 Jan 18;20(1):167-180. doi: 10.1007/s40201-021-00764-0. PMID: 35669826; PMCID: PMC9163233.
Highlights
- Biofilm formation and antibiotic resistance are the most important ways in which water bacteria such as Pseudomonas aeruginosaare protected against antibacterial agents.
- The aim of this study was to develop a rapid and cost-effective laboratory method for modeling and optimizing chlorine disinfection conditions.
- Critical factors (disinfection type, concentration, contact time and pH) were tested on bactericidal effect, inhibition of biofilm formation (IBF) and antibiotic susceptibility (AS) of aeruginosa.
- Optimization of factors affecting disinfection had a significant effect on the planktonic form, but was not effective in removing the biofilm of aeruginosa.
- The use of chlorine compounds based on the recommended levels in water does not prevent the formation of aeruginosabiofilm.
- Increasing the contact time and concentration of the disinfectant increases the bactericidal effect of chlorine, it can also increase the resistance of aeruginosato some antibiotics.